Afjal Miah
Seminars
Wednesday 11th March 2026
High Throughput Synthesis Strategies for Targeted Protein Degradation Applications
2:30 pm
- Overview of an integrated high-throughput workflow combining DEL screening and – Direct‑to‑Biology (D2B) chemistry to rapidly discover E3 ligase binders and assess their PROTAC potential through high‑throughput synthesis and cellular degradation assays
- Case studies (KLHDC2 and a second novel E3 ligase) demonstrating mechanism‑validated degradation across multiple protein targets and the experimental approaches used to confirm on‑target activity
- Practical lessons learned and comparisons of D2B versus purified‑compound workflows, including advantages, limitations, and implications for hit triage and lead optimization
